purelink viral rna dna mini kit (Thermo Fisher)

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Thermo Fisher purelink viral rna dna mini kit
Purelink Viral Rna Dna Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/purelink+rna+mini+kit/Deoxyribonucleic+acid/pm41641994-8-5-10
Average 99 stars, based on 1 article reviews

purelink viral rna dna mini kit - by Bioz Stars, 2026-10

99/100 stars

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Expressing:

Article Title: Lipoyl deglutarylation by ABHD11 regulates mitochondrial and T cell metabolism.
Article Snippet: .. To analyze mRNA expression, total RNA was collected from cells using the PureLink RNA Mini Kit (Thermo Fisher Scientific), and 1 μg was reverse-transcribed using the ProtoScript II First Strand cDNA Synthesis Kit with oligo(dT) primers and murine RNase inhibitor (New England Biolabs). .. Quantitative PCR was performed in technical triplicate using 20 ng cDNA, 1× Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) and 0.5 μM forward and reverse primers (Supplementary Table 1), using an ABI 7900HT Real-Time PCR System (Applied Biosystems).

Reverse Transcription:

Article Title: M2-like macrophages derived from THP-1 cells promote myofibroblast differentiation of synovial fibroblasts in association with the TGF-β1/SMAD2/3 signaling pathway
Article Snippet: Phase contrast images of cells were captured using an Eclipse TE300 inverted microscope (Nikon, Tokyo, Japan). .. For the confirmation of differentiation by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), total RNA was extracted using a PureLink RNA Mini Kit (Thermo Fisher Scientific, Waltham, MA, USA). cDNA synthesis was performed using a High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific, Waltham, MA, USA) with a GeneAmp PCR System 9700 thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA). .. RT-qPCR analyses were performed using the iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) with the CFX Connect (Bio-Rad, Hercules, CA, USA).

Article Title: M2-like macrophages derived from THP-1 cells promote myofibroblast differentiation of synovial fibroblasts in association with the TGF-β1/SMAD2/3 signaling pathway.
Article Snippet: Phase contrast images of cells were captured using an Eclipse TE300 inverted microscope (Nikon, Tokyo, Japan). .. For the confirmation of differentiation by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), total RNA was extracted using a PureLink RNA Mini Kit (Thermo Fisher Scientific, Scientific Reports | (2025) 15:25505 2| https://doi.org/10.1038/s41598-025-10858-6 Waltham, MA, USA). cDNA synthesis was performed using a High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific, Waltham, MA, USA) with a GeneAmp PCR System 9700 thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA). .. RT-qPCR analyses were performed using the iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) with the CFX Connect (Bio-Rad, Hercules, CA, USA).

cDNA Synthesis:

Article Title: M2-like macrophages derived from THP-1 cells promote myofibroblast differentiation of synovial fibroblasts in association with the TGF-β1/SMAD2/3 signaling pathway.
Article Snippet: Phase contrast images of cells were captured using an Eclipse TE300 inverted microscope (Nikon, Tokyo, Japan). .. For the confirmation of differentiation by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), total RNA was extracted using a PureLink RNA Mini Kit (Thermo Fisher Scientific, Scientific Reports | (2025) 15:25505 2| https://doi.org/10.1038/s41598-025-10858-6 Waltham, MA, USA). cDNA synthesis was performed using a High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific, Waltham, MA, USA) with a GeneAmp PCR System 9700 thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA). .. RT-qPCR analyses were performed using the iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) with the CFX Connect (Bio-Rad, Hercules, CA, USA).

Cell Culture:

Article Title: An osteoblast-like cell line derived from mice expressing FRET-based tension sensor reveals cellular tension increase during osteogenic differentiation
Article Snippet: .. Cells cultured in OM for 2 or 7 days were lysed with ISOGEN II (Nippon Gene, Japan) for RNA extraction using the PureLink RNA Mini Kit (Thermo Fisher). .. RNA concentration was measured with a NanoDrop One spectrophotometer (Thermo Fisher).

RNA Extraction:

Article Title: Impact of Nanoparticle Stiffness on Endosomal Escape and Signaling Pathways in Cytosolic Delivery.
Article Snippet: After final suspension, samples were analyzed using the BD FACSVia flow cytometry (mRNA-FITC/GFP filter, DNA-PE filter, US), and data were processed using FlowJo software (v10.10.0). .. RNA Extraction: Total RNA was extracted using the PureLink RNA Mini Kit (Thermo Fisher Scientific) following the manufacturer’s instructions. .. The purity of the extracted RNA was assessed by NanoPhotometer NP80 (IMPLEN, Germany), with an A260/280 ratio of ∼2.0 considered indicative of purified RNA.

Quantitative RT-PCR:

Article Title: A shade-hyposensitive tomato line shows altered auxin homeostasis and higher fruit yield under high-density field conditions.
Article Snippet: .. RT-qPCR analyses Total RNA for RT-qPCR was isolated from frozen leaf samples using the Purelink RNA mini kit (Thermo Fisher Scientific, Waltham MA, USA). ..

Isolation:

Polymerase Chain Reaction:

Article Title: M2-like macrophages derived from THP-1 cells promote myofibroblast differentiation of synovial fibroblasts in association with the TGF-β1/SMAD2/3 signaling pathway.
Article Snippet: Phase contrast images of cells were captured using an Eclipse TE300 inverted microscope (Nikon, Tokyo, Japan). .. For the confirmation of differentiation by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), total RNA was extracted using a PureLink RNA Mini Kit (Thermo Fisher Scientific, Scientific Reports | (2025) 15:25505 2| https://doi.org/10.1038/s41598-025-10858-6 Waltham, MA, USA). cDNA synthesis was performed using a High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific, Waltham, MA, USA) with a GeneAmp PCR System 9700 thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA). .. RT-qPCR analyses were performed using the iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) with the CFX Connect (Bio-Rad, Hercules, CA, USA).

Incubation:

Article Title: Angiotensin AT 1 R expressing cells within the ventral hypothalamus modulate integrative control of cardiometabolic functions
Article Snippet: .. The supernatant was combined equally with chloroform, vortexed, incubated at room temperature for ∼3 min, and centrifuged at 4°C at 12,000xg for 15 min. Total RNA was purified from the aqueous layer following the PureLink RNA Mini Kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s protocol. .. RNA was quantified by a NanoDrop 1000 Spectrophotometer (ThermoFisher Scientific, Waltham, MA) and stored at −80°C.

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